IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/03
From OpenWetWare
Current revision (14:50, 12 June 2008) (view source) |
|||
| Line 36: | Line 36: | ||
| - | [[Image:UW_AAT.jpg|350px]] | + | [[Image:UW_AAT.jpg|350px]] [[Image:UW_T.jpg|350px]] |
Current revision
 University of Warsaw iGEM Team 2008
|  Main project page Previous entry Next entry
|
|
2. Digest with HindIII and SalI 3. Digest of pMPM-T5 + AID with HindIII and SalI 4. Clean-up products of p.2 and p.3 reactions 5. Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion) 6. Transformation of E.coli TOP10 with ligation product 7. Transformants onto LB+tetracycline 8. Digest of pMPM-T5 + transcription fusion with EcoRHI and HindIII 9. Use of T4 polymerase for blunting 10. Gel electrophoresis 11. Isolation from proper band 12. Ligation of product p.11 (pMPM-T5 + T7 RNA-polimerase) 13. Transformation of E.coli TOP10 with ligation product 14. Transformants onto LB + tetracycline
| |
