IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/03: Difference between revisions
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Revision as of 11:50, 12 June 2008
 University of Warsaw iGEM Team 2008
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2. Digest with HindIII and SalI 3. Digest of pMPM-T5 + AID with HindIII and SalI 4. Clean-up products of p.2 and p.3 reactions 5. Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion) 6. Transformation of E.coli TOP10 with ligation product 7. Transformants onto LB+tetracycline 8. Digest of pMPM-T5 + transcription fusion with EcoRHI and HindIII 9. Use of T4 polymerase for blunting 10. Gel electrophoresis 11. Isolation from proper band 12. Ligation of product p.11 (pMPM-T5 + T7 RNA-polimerase) 13. Transformation of E.coli TOP10 with ligation product 14. Transformants onto LB + tetracycline
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