IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/06: Difference between revisions
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(Autocreate 2008/06/06 Entry for IGEM:UW/2008/Notebook/iGEM_UW_Team) |
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== | ==PCR pZCseq== | ||
program to amplify fragment of pZC320 for sequencing | |||
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PCR program: iGEMpZCseq | |||
polymerase: Pfu Turbo | |||
step time temp | |||
initial denaturation 03:00 95 | |||
denaturation 00:30 95 30 | |||
annealing 00:45 47 | |||
elongation 01:30 72 | |||
final elongation 10:00 72 | |||
freeze forever 4 | |||
Revision as of 02:58, 6 June 2008
 University of Warsaw iGEM Team 2008
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PCR pZCseqprogram to amplify fragment of pZC320 for sequencing
PCR program: iGEMpZCseq polymerase: Pfu Turbo step time temp initial denaturation 03:00 95 denaturation 00:30 95 30 annealing 00:45 47 elongation 01:30 72 final elongation 10:00 72 freeze forever 4
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