IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/06

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==Entry title==
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==PCR pZCseq==
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program to amplify fragment of pZC320 for sequencing
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PCR program: iGEMpZCseq
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polymerase: Pfu Turbo
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step                 time    temp
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initial denaturation 03:00 95
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denaturation         00:30 95 30
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annealing         00:45 47
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elongation         01:30 72
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final elongation 10:00 72
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freeze                 forever 4

Revision as of 04:58, 6 June 2008

University of Warsaw iGEM Team 2008 Main project page
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PCR pZCseq

program to amplify fragment of pZC320 for sequencing

PCR program: iGEMpZCseq polymerase: Pfu Turbo

step time temp initial denaturation 03:00 95 denaturation 00:30 95 30 annealing 00:45 47 elongation 01:30 72 final elongation 10:00 72 freeze forever 4



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