IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/19

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(Autocreate 2008/06/19 Entry for IGEM:UW/2008/Notebook/iGEM_UW_Team)
Current revision (13:22, 26 June 2008) (view source)
 
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Primers for fusion of protein A and ompA and alpha or omega part of beta-lactamase were designed:
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alphaL+link14+homo CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CACCCAGAAACGCTGGTGAAAG
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alphaP+STOP+XbaI+BamHI GG GGATCC GG TCTAGA TTA TTCGCCAGTTAATAGTTTGCG
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omegaL+link14+homo CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CTACTTACTCTAGCTTCCCGG
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omegaP+STOP+EcoRI+PstI+BamHI AAGGATCCAACTGCAGAAGAATTC TTACCAATGCTTAATCAGTG
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OmpAL+NdeI CATATGAAAGCTACTAAACTGGTACTGGG
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OmpAP+link8+SacI+NotI+homo GCCACCGCTTCCACCACT GCGGCCGC CA GAGCTC CCTCCTCCAGAACCTCC GCCGTTGTCCGGACGAGTGCCGATG

Current revision

University of Warsaw iGEM Team 2008 Main project page
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Primers for fusion of protein A and ompA and alpha or omega part of beta-lactamase were designed:

alphaL+link14+homo CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CACCCAGAAACGCTGGTGAAAG

alphaP+STOP+XbaI+BamHI GG GGATCC GG TCTAGA TTA TTCGCCAGTTAATAGTTTGCG

omegaL+link14+homo CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CTACTTACTCTAGCTTCCCGG

omegaP+STOP+EcoRI+PstI+BamHI AAGGATCCAACTGCAGAAGAATTC TTACCAATGCTTAATCAGTG

OmpAL+NdeI CATATGAAAGCTACTAAACTGGTACTGGG

OmpAP+link8+SacI+NotI+homo GCCACCGCTTCCACCACT GCGGCCGC CA GAGCTC CCTCCTCCAGAACCTCC GCCGTTGTCCGGACGAGTGCCGATG



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