IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/06/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Paraoxon Biosensor 1</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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Revision as of 16:04, 29 June 2009
Paraoxon Biosensor 1 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Primers
Deletion constructsConstruction of YGR035C deletion, replacement by GFP via homologous gene recombination in S. cerevisiae PCR YGR035Cdel-F/YGR035Cdel-R on PCR YGR035CFor/YGR035CRev on pFA6a-GFP(S65T)-kanMX6 (2504bp, EcoRI/SpeI/DpnI) Sub into YGR035C gene on Chr. VII Product is delYGR035C-GFP [KanR]
PCR YHR139Cdel-F/YHR139Cdel-R on pFA6a-GFP(S65T)-kanMX6 (2504bp, EcoRI/SpeI/DpnI) Sub into YHR139C gene on Chr. VIII Product is delYHR139C-GFP [KanR]
PCR YOR049Cdel-F/YOR049del-R on pFA6a-GFP(S65T)-kanMX6 (2504bp, EcoRI/SpeI/DpnI) Sub into YOR049C gene on Chr. XV Product is delYOR039-GFP [KanR]
PCR YOR186WCdel-F/YOR186Wdel-R on pFA6a-GFP(S65T)-kanMX6 (2504bp, EcoRI/SpeI/DpnI) Sub into YOR186W gene on Chr. XV Product is delYOR186W-GFP [KanR]
PCR YGR213Cdel-F/YGR213Cdel-R on pFA6a-GFP(S65T)-kanMX6 (2504bp, EcoRI/SpeI/DpnI) Sub into YGR213C gene on Chr. VII Product is delYGR213C-GFP [KanR]
Construction of YGR236C deletion, replacement with GFP via homologous gene recombination in S. cerevisiae PCR YGR236Cdel-F/YGR236Cdel-R on pFA6a-GFP(S65T)-kanMX6 (2504bp, EcoRI/SpeI/DpnI) Sub into YGR236C gene on Chr. VII Product is delYGR236C-GFP [KanR]
PCR YLR346Cdel-F/YLR346del-R on pFA6a-GFP(S65T)-kanMX6 (2504bp, EcoRI/SpeI/DpnI) Sub into YLR346C gene on Chr. XII Product is delYLR346C-GFP [KanR]
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