IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/07/01: Difference between revisions
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* After running PCR, realized we mixed up primers/templates for 139 and 186; they were discarded. | * After running PCR, realized we mixed up primers/templates for 139 and 186; they were discarded. | ||
* Ran a gel; only got funky bands at 850kb; no actual product | * Ran a gel; only got funky bands at 850kb; no actual product | ||
Top: Ladder, -DNA, -Pol, 035, -, 49, 213, -, 236, 346. At 55C 41(0), 49(1), 49(2), 49(4) | |||
Bottom: Ladder, -, -, 035, 49, 186, 213, 236, 346 | |||
*[[Image:2009-07-01.FragmentPcrAll.jpg]] | |||
* must be a mispriming error, which is weird because they are supposedly no redundancies according to the sequence | * must be a mispriming error, which is weird because they are supposedly no redundancies according to the sequence | ||
Revision as of 08:12, 6 July 2009
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July 1, 2009PCR
Restriction Digest
Gel Purification
Top: Ladder, -DNA, -Pol, 035, -, 49, 213, -, 236, 346. At 55C 41(0), 49(1), 49(2), 49(4) Bottom: Ladder, -, -, 035, 49, 186, 213, 236, 346
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