IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/07/15

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July 15th, 2009

Agarose Gel for PCR Confirmation

Purpose: Ran gel to verify the correction amplification of desired products. Desired fragments should be 2.3 kb in length, according to the Langtine paper. Gel visualization is included below. All five PCR reations produced the desired fragment length. Controls were proper. DNA ladder concentration was low, but showed up on computer, not on printed copy.

Qubit Fluorometer DNA Concentration of PCR Products Ran Qubit Assay to check concentration of DNA for reference after transformations.

  1. YJL219 -> Reading = 5.7, Concentration = 1.14 ug/ml
  2. YGR287 -> Reading = 6.0, Concentration = 1.20 ug/ml
  3. YDL243 -> Reading = 6.0, Concentration = 1.20 ug/ml
  4. YHL012 -> Reading = 5.7, Concentration = 1.14 ug/ml
  5. YGL205 -> Reading = 5.2, Concentration = 1.04 ug/ml

Using 10 uL of DNA per transformation, I would be using 0.01 mg or 100 ug of DNA per transformation. 2 uL DNA -> 2 ug DNA



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