IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/07/30: Difference between revisions
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(Autocreate 2009/07/30 Entry for IGEM:University_of_Chicago/2009/Notebook/Paraoxon_Biosensor) |
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== | ==July 30th, 2009== | ||
Fluorescence and Viability Assay | |||
Take overnight cells, dilute to 0.05, and have both a non-PNP control and a PNP at 1 mM test. Do this for both 4.1 and 4.2 strains. | |||
Below is a chart for the assay results. Induced with PNP at 1:48 p.m. Might see a decline in growth rate. Maybe glowing, also. 1mM | |||
4.1 cont | |||
OD 1:30 = 0.152, OD 3:00 = 0.242, OD 4:30 = 0.438, OD 6:00 = 0.641 | |||
4.1 PNP | |||
OD 1:30 = 0.148, OD 3:00 = 0.210, OD 4:30 = 0.260, OD 6:00 = 0.355 | |||
4.2 cont | |||
OD 1:30 = 0.284, OD 3:00 = 0.209, OD 4:30 = 0.465, OD 6:00 = 0.635 | |||
4.2 PNP | |||
OD 1:30 = 0.131, OD 3:00 = 0.273, OD 4:30 = 0.224, OD 6:00 = 0.419 | |||
Trans 4 | |||
OD 1:30 = 0.472 | |||
Trans 4 | |||
OD 1:30 = 0.421 | |||
Transformation 4 (longtine PCR fragments) | |||
- Attempt to transform Longtine PCR fragments into SKY816. OD average of two (10 ml) cultures was approximately 0.44. Hopefully, all will transform. If not all, hopefully fragments 1 and 5. | |||
-PEG mixture seems a bit viscous. Not sure if it's correct or not.... | |||
Revision as of 13:51, 20 October 2009
 iGEM Project name 1
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July 30th, 2009Fluorescence and Viability Assay Take overnight cells, dilute to 0.05, and have both a non-PNP control and a PNP at 1 mM test. Do this for both 4.1 and 4.2 strains. Below is a chart for the assay results. Induced with PNP at 1:48 p.m. Might see a decline in growth rate. Maybe glowing, also. 1mM
OD 1:30 = 0.152, OD 3:00 = 0.242, OD 4:30 = 0.438, OD 6:00 = 0.641 4.1 PNP OD 1:30 = 0.148, OD 3:00 = 0.210, OD 4:30 = 0.260, OD 6:00 = 0.355 4.2 cont OD 1:30 = 0.284, OD 3:00 = 0.209, OD 4:30 = 0.465, OD 6:00 = 0.635 4.2 PNP OD 1:30 = 0.131, OD 3:00 = 0.273, OD 4:30 = 0.224, OD 6:00 = 0.419 Trans 4 OD 1:30 = 0.472 Trans 4 OD 1:30 = 0.421
- Attempt to transform Longtine PCR fragments into SKY816. OD average of two (10 ml) cultures was approximately 0.44. Hopefully, all will transform. If not all, hopefully fragments 1 and 5. -PEG mixture seems a bit viscous. Not sure if it's correct or not....
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