IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/08/03: Difference between revisions
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* very thick band seen at ~750 | * very thick band seen at ~750 | ||
*will do restriction digest/ligation tomorrow | *will do restriction digest/ligation tomorrow | ||
==August, 3rd, 2009 P2== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''OD660 Time''' | |||
| align="center" style="background:#f0f0f0;"|'''12:20''' | |||
| align="center" style="background:#f0f0f0;"|'''2:20''' | |||
| align="center" style="background:#f0f0f0;"|'''4:20''' | |||
| align="center" style="background:#f0f0f0;"|'''6:20''' | |||
| align="center" style="background:#f0f0f0;"|'''7:50''' | |||
| align="center" style="background:#f0f0f0;"|'''10:10''' | |||
| align="center" style="background:#f0f0f0;"|'''11:20''' | |||
|- | |||
| YGR035 Cont||0.186||0.222||.283||.242||0.436||0.882||1.213|| | |||
|- | |||
| YGR035 0.5 mM||0.183*||0.158||.137||.202||0.185||0.190||0.382|| | |||
|- | |||
| YGR346 Cont||0.171||0.301||.412||.974||1.252||1.383||1.478|| | |||
|- | |||
| YGR346 0.5mM||0.204*||0.289||.187||.223||0.277||0.270||0.444|| | |||
|- | |||
| 1 Cont||0.366||0.416||.492||.975||1.331||1.452||1.427|| | |||
|- | |||
| 1 0.5 mM||0.166*||0.408||.388||.618||1.029||1.218||1.346|| | |||
|- | |||
| 2 Cont||0.168||0.337||.283||.324||0.490||0.898||1.223|| | |||
|- | |||
| 2 0.5mM||0.189*||0.270||.396||.462||0.746||1.102||1.245|| | |||
|- | |||
| 3 Cont||0.237||0.256||.247||.448||0.481||0.887||1.183|| | |||
|- | |||
| 3 0.05 mM||0.274*||0.396||.330||.467||0.590||1.102||1.314|| | |||
|- | |||
| 3 0.1 mM||0.126*||0.225||.263||.276||0.482||1.008||1.274|| | |||
|- | |||
| 3 0.25 mM||0.239*||0.251||.232||.373||0.647 (0.697?)||1.140||1.305|| | |||
|- | |||
| 3 0.5 mM||0.232*||0.349||.160||.281||0.408||0.720||1.053|| | |||
|- | |||
| 4 Cont||0.229||0.265||.319||.362||0.371||0.586||0.959|| | |||
|- | |||
| 4 0.5 mM||0.293*||0.236||.226||.281||0.319||0.236||0.277|| | |||
| | |||
Added to 4.5 ml cultures | |||
0.5 mM = 22 uL | |||
0.1 mM = 4.1 uL | |||
0.05 mM = 2.2 uL | |||
0.25 mM = 11 uL | |||
* = time of induction | |||
Visual Test for Glow | |||
1.(0.5) Inconclusive: no green glows | |||
2.(0.5) "" | |||
3. (0.1) "" | |||
3. (0.25) "" | |||
3. (0.5) "" | |||
4. (0.5) "" | |||
035 (0.5) "" | |||
346 (0.5) "" | |||
Glowing tests did not confirm any glowing yeast cultures. | |||
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Revision as of 13:52, 20 October 2009
iGEM Project name 1 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
August 3, 2009
Concentrations of DNA + dilution: template-RFP: 7.42 ng/μL diluted to 74 pg/μL template-mcherry: 326 ng/μL diluted to 65.0 pg/μL
For both mcherry and RFPTurbo beads: 2μL 5' primer: 5μL 3' primer: 5μL template: 2μL ddH2O: 36μL Total: 50μL Program name - RFP Incubation: 95°C 5 minutes Separation: 95°C 30 seconds Annealing: 58°C 30 seconds Extension: 72°C 1.5 minutes Repeat x30
2 beads 5' primers: 5μL 3' primers: 5μL temp 2μL ddH2O 46: 38μL
August, 3rd, 2009 P2
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