IGEM:University of Chicago/2009/Notebook/Paraoxon Biosensor/2009/08/13: Difference between revisions
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(Autocreate 2009/08/13 Entry for IGEM:University_of_Chicago/2009/Notebook/Paraoxon_Biosensor) |
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== | ==August 13th, 2009== | ||
measured DNA conc. using nanodrop appartus. this DNA was cut using PacI and AscI (overnight) and then gel purified. | |||
Ligation (super GFP into Longtine Plasmids [Kan and His]) | |||
20 ul RXNs | |||
4 5 x buffer | |||
2. plasmid | |||
2. insert | |||
1 igase | |||
11 ddH2O | |||
Ran four reactions | |||
1. S-GFP + His Plasmid | |||
2. S-GRFP + kan plasmid | |||
Revision as of 13:55, 20 October 2009
 iGEM Project name 1
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August 13th, 2009measured DNA conc. using nanodrop appartus. this DNA was cut using PacI and AscI (overnight) and then gel purified. Ligation (super GFP into Longtine Plasmids [Kan and His]) 20 ul RXNs 4 5 x buffer 2. plasmid 2. insert 1 igase 11 ddH2O Ran four reactions 1. S-GFP + His Plasmid 2. S-GRFP + kan plasmid
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