IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/06/19: Difference between revisions

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Today in the lab we ran a gel of the restriction digest products from the previous day. Upon inspection of the gel, it was not clear which band contained the DNA we needed to extract. We had expected to see two bands for both the iGEM and antGPneo plasmids, but there were three. This meant the incorrect band was chosen to extract the large fragment DNA from the iGEM plasmid digest. After discussion, it was decided that the best option would be to perform the digests again and run the gel for those on the following morning.  
Today in the lab we ran a gel of the restriction digest products from the previous day. Upon inspection of the gel, it was not clear which band contained the DNA we needed to extract. We had expected to see two bands for both the iGEM and antGPneo plasmids, but there were three. This meant the incorrect band was chosen to extract the large fragment DNA from the iGEM plasmid digest. After discussion, it was decided that the best option would be to perform the digests again and run the gel for those on the following morning.  


At 4pm, there was a team meeting where we produced a general agenda for all following team meetings. The rest of the team were told of lab developments on floor two and we were excited to hear of the sediments samples we were going to recieve from south china sea and the north alantic ocean, after our successful application to a french lab group.  
At 4pm, there was a team meeting where we produced a general agenda for all following team meetings. The rest of the team were told of lab developments on floor two and we were excited to hear of the sediments samples we were going to recieve from south china sea and the north alantic ocean, after our successful application to a french lab group. There was also some discussion on the ethics of recieving soil and sediment samples.  




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Today in the lab we ran a gel of the restriction digest products from the previous day. Upon inspection of the gel, it was not clear which band contained the DNA we needed to extract. We had expected to see two bands for both the iGEM and antGPneo plasmids, but there were three. This meant the incorrect band was chosen to extract the large fragment DNA from the iGEM plasmid digest. After discussion, it was decided that the best option would be to perform the digests again and run the gel for those on the following morning.

At 4pm, there was a team meeting where we produced a general agenda for all following team meetings. The rest of the team were told of lab developments on floor two and we were excited to hear of the sediments samples we were going to recieve from south china sea and the north alantic ocean, after our successful application to a french lab group. There was also some discussion on the ethics of recieving soil and sediment samples.



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