IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/06/26: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Four</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Four</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Cultures grown overnight were retrieved and DNA mini preps was performed. This was followed by restriction digest reactions using EcoR1 and Pst1 enzymes. Samples were then run on an agarose gel to confirm the digest reactions. | Cultures grown overnight were retrieved and DNA mini preps was performed. This was followed by restriction digest reactions using EcoR1 and Pst1 enzymes. Samples were then run on an agarose gel, ''fig 1,'' to confirm the digest reactions. | ||
[[Image:26 | [[Image:800px-26 june.jpg|right|thumb|Fig 1:Analysis of restriction digest products of ligated, transformed plasmids using agrose gel electrophoresis. Samples shown are the control (original J04450 plasmid), and colonies 1-6 respectively) | ||
.]].Also, it was today in which we discovered and confirmed that our AntgP Neo sequence was wrong, missing the Xba1 restriction site. So we had to do further planning for potential experiments. | |||
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Latest revision as of 23:04, 26 September 2017
 Week Four
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Cultures grown overnight were retrieved and DNA mini preps was performed. This was followed by restriction digest reactions using EcoR1 and Pst1 enzymes. Samples were then run on an agarose gel, fig 1, to confirm the digest reactions. 
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