IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/09: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Six</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Six</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Floor Two== | |||
PCR samples were retrieved and run on a gel ''fig 1'', This was followed by the transformation of samples into bacterial cells, which was performed following the kit's protocol. Samples were plated on agar plates and incubated overnight at 37°C | |||
For the protein experiments, a colony from each of the four transformation plates was inoculated into LB (2 samples with Kanamycin and Chlorophenicol, 2 samples with Kanamycin only) and left to grow overnight at 37°C in a shaking incubator. | |||
[[Image:9 july 2.jpg|thumb|Fig 1: PCR results run on agarose gel electrophoresis, where sample 1 is the control with no DNA added, sample 2 is the AntGp-Neo-Xba1 biobrick and sample 3 is the J04450-Nde1 biobrick]] | |||
Latest revision as of 23:03, 26 September 2017
 Week Six
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Floor TwoPCR samples were retrieved and run on a gel fig 1, This was followed by the transformation of samples into bacterial cells, which was performed following the kit's protocol. Samples were plated on agar plates and incubated overnight at 37°C
For the protein experiments, a colony from each of the four transformation plates was inoculated into LB (2 samples with Kanamycin and Chlorophenicol, 2 samples with Kanamycin only) and left to grow overnight at 37°C in a shaking incubator. 
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