IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/25: Difference between revisions
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==Floor Two== | ==Floor Two== | ||
The two ligation samples from the previous day were analysed by gel electrophoresis alongside controls and the samples from the 22nd July. | |||
[[Image:BIORAD 2013-07-25 15hr 59min.JPG|thumb|Fig. Analysis of Ligation reactions by gel electrophoresis. Lane 1 and 2 (24/07 ligations), lanes 3 and 4 controls, lane 5 and 6 samples from 22nd July (2b and 2c).]] | |||
The membrane was retrieved from the shaker and washed with TBST and TBS solutions respectively for ten minutes. It was then incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result. | The membrane was retrieved from the shaker and washed with TBST and TBS solutions respectively for ten minutes. It was then incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result. | ||
Revision as of 07:36, 31 July 2013
 Week 8
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Floor TwoThe two ligation samples from the previous day were analysed by gel electrophoresis alongside controls and the samples from the 22nd July.  The membrane was retrieved from the shaker and washed with TBST and TBS solutions respectively for ten minutes. It was then incubated in Anti His HRP conjugate solution. After further washing the membrane was imaged using chemiluminescent detection. This gave no visible result.
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