IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/29

From OpenWetWare
Jump to navigationJump to search
Week Nine <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Floor 1

Streaked lawns of single Streptomyces colonies that appeared to be sporulating and had large clear single colonies.

Re-streaked contaminated single Streptomyces colony plates.

Streaked from the plates in the conjugated Steptomyces trial that had grown on the kanamycin plates (?).

Floor 2

Restriction digest reactions were performed on the original J04450 original plasmid and the J04450-Nde1 bio brick using (i) EcoR1 and Pst1 and (ii) Nde1 and Xba1. After incubation samples were run on agarose gel fig 5. Transformation was done on the AntgP-Neo biobrick (ligated samples 2b and 2c). Also, colonies 1 and 2 (used in colony PCR) from samples 1 and 2 were inoculated and grown overnight.

Fig 5:Restriction digest of J04450 original plasmid (lanes 1 and 3) and J04450-Nde1 biobrick (lanes 2 and 4) using i) EcoR1 and Pst1 and (ii) Nde1 and Xba1.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM/2013/07/29&oldid=718673"