IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/01: Difference between revisions
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==Floor Two== | ==Floor Two== | ||
The Biobrick AntAgp+Xba+Neo was digested by restriction enzymes Xba1 and Nde1. The samples were then analysed by gel electophoresis, but after imaging | The Biobrick AntAgp+Xba+Neo was digested by restriction enzymes Xba1 and Nde1. The samples were then analysed by gel electophoresis, but after imaging the results of the digestion were unclear. | ||
The protein over expression was continued by inoculating two new LB solutions (1a,1b) with 100μL from the overnight culture. 40μL of IPTG was added to sample 1b and both cultures were left to incubate at 25°C overnight. | The protein over expression was continued by inoculating two new LB solutions (1a,1b) with 100μL from the overnight culture. 40μL of IPTG was added to sample 1b and both cultures were left to incubate at 25°C overnight. | ||
Revision as of 06:18, 8 August 2013
 Week Nine
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Floor TwoThe Biobrick AntAgp+Xba+Neo was digested by restriction enzymes Xba1 and Nde1. The samples were then analysed by gel electophoresis, but after imaging the results of the digestion were unclear. The protein over expression was continued by inoculating two new LB solutions (1a,1b) with 100μL from the overnight culture. 40μL of IPTG was added to sample 1b and both cultures were left to incubate at 25°C overnight.
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