IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/06
From OpenWetWare
iGEM Project name 1 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Floor OneMade 1 % agarose gel - 2 x large - 2 x 100 ml TBE buffer and 1 g agarose. Melted in microwave til agarose dissolved. Prepared spore stocks of all lawns. Did as much lawning and streak purifying as was possile with available SFM + Ny media. Made 6 x 500 ml SFM agar, autoclaved for 1 hr and poured into plates. Loaded and ran gels - added 4 ul loading dye to each sample (46). Used 2 x ladder (1 Kb). Made up more liquid Nystatin (50 mg/ml) - 10 ml DMSO and 500 mg Nystatin.
|