IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/15: Difference between revisions
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==Floor One== | |||
Made 4 x 500 ml and 2 x 1000 ml of SFM + Ny. | |||
Completed more lawns of single Streptomyces colonies. | |||
Decided to no longer re-streak contaminated plates as we have sufficient plates growing to produce a reasonable no. of spore stocks. | |||
==Floor Two== | ==Floor Two== | ||
The two plates and control plate from the previous day were successful, so two colonies were picked from plate one and used to inoculate two 10ml LB solutions and left to incubate overnight at 37°C. <br> | The two plates and control plate from the previous day were successful, so two colonies were picked from plate one and used to inoculate two 10ml LB solutions and left to incubate overnight at 37°C. <br> | ||
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Two LB solutions were inoculated with BL21 PlySs and BL21 Rosetta PlySs seperately and incubated overnight at 37°C. | Two LB solutions were inoculated with BL21 PlySs and BL21 Rosetta PlySs seperately and incubated overnight at 37°C. | ||
[[Image:Bl21 Anta plates.jpg|Fig.1: | [[Image:Bl21 Anta plates.jpg|thumb|Fig.1:Transformation of pETAntA plasmid into BL21 ''E.Coli'' cells and spread onto LB agar + Kanamycin plates.]] | ||
Revision as of 07:46, 22 August 2013
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Floor OneMade 4 x 500 ml and 2 x 1000 ml of SFM + Ny. Completed more lawns of single Streptomyces colonies. Decided to no longer re-streak contaminated plates as we have sufficient plates growing to produce a reasonable no. of spore stocks.
Floor TwoThe two plates and control plate from the previous day were successful, so two colonies were picked from plate one and used to inoculate two 10ml LB solutions and left to incubate overnight at 37°C.
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