IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Eleven</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Eleven</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Floor One== | |||
De-replicated single Streptomyces colony plates when it appeared that we had duplicates. This is spore stock the minimum amount of lawns. | |||
Filtered, concentrated and resuspended spore stocks in 20 % glycerol. | |||
==Floor Two== | ==Floor Two== | ||
The two overnight cultures, containing BL21 cells that had been transformed with Pet28AntA were retrieved. Culture 1a was used to make competent cells, while sample 1b was induced with IPTG and left to incubate at 25°C overnight. While the cells were growing, minipreps of BL21 PlySs and BL21 rosetta PlySs cultures were prepared and stored in the freezer. <br> | The two overnight cultures, containing BL21 cells that had been transformed with Pet28AntA were retrieved. Culture 1a was used to make competent cells, while sample 1b was induced with IPTG and left to incubate at 25°C overnight. While the cells were growing, minipreps of BL21 PlySs and BL21 rosetta PlySs cultures were prepared and stored in the freezer. <br> | ||
The BL21 Pet28AntA competent cells were transformed with the biobrick BBa_K1041002 (AntG promoter + RFP gene) and spread onto a LB Agar plate containing Kanamycin and Chlorophenicol antibiotics and six control plates were prepared, ''fig 2'' | The BL21 Pet28AntA competent cells were transformed with the biobrick BBa_K1041002 (AntG promoter + RFP gene) and spread onto a LB Agar plate containing Kanamycin and Chlorophenicol antibiotics and six control plates were prepared, ''fig 2'' | ||
[[Image:BL21 plates 16-08.JPG|thumb|Fig 2: A table to show what each LB Agar plate contained.]] | [[Image:BL21 plates 16-08.JPG|thumb|Fig 2: A table to show what each LB Agar plate contained.]] | ||
==Outreach== | |||
A meeting was held to discuss the upcoming forum event such as posters and layout for the tables. | |||
Latest revision as of 23:11, 26 September 2017
 Week Eleven
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Floor OneDe-replicated single Streptomyces colony plates when it appeared that we had duplicates. This is spore stock the minimum amount of lawns. Filtered, concentrated and resuspended spore stocks in 20 % glycerol. Floor TwoThe two overnight cultures, containing BL21 cells that had been transformed with Pet28AntA were retrieved. Culture 1a was used to make competent cells, while sample 1b was induced with IPTG and left to incubate at 25°C overnight. While the cells were growing, minipreps of BL21 PlySs and BL21 rosetta PlySs cultures were prepared and stored in the freezer.  OutreachA meeting was held to discuss the upcoming forum event such as posters and layout for the tables.
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