IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Thirteen</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Floor One==
==Outreach==
Today we held our public engagement event at the forum in Norwich city centre. six members of our team attended the event which lasted all day. There were flags in maps of Europe and Norfolk to highlight where we had received soil samples from and freebies of pens, sweets and general science information. We had various posters and leaflets to explain about iGEM and our project. <br>
The main attraction was biscuit decorating, which involved recreating a petri dish of bacteria on a biscuit using icing and sweets. We could then explain to the children and their parents about ''Streptomyces'' and antibiotic resistance. It was a very successful event with alot of interest.
[[Image:994568 157275804469334 23825595 n.jpg|thumb|Bacteria Biscuit making at Forum event]]


To gain an idea of what strains of Streptomyces are growing on the plates, liquid cultures of the purified streaked Streptomyces plates for 16S sequencing were prepared by transferring colonies to LB (-NaCl) broth + a spring to aerate the cultures.
Started preparing spore stocks by adding approx. 2 ml glycerol to lawn plates and using a cotton bud to shift the spores, adding more glycerol if required. The resulting liquid was then filtered using cotton wool in a 5 ml syringe. We spore stocked 32 lawns, 35 are remaining atm.
Liquid cultures were set up for E. coli ETpuz cells - LB broth was inoculated with cultures from pAU3-45 and pMS82 plates. The pAU3-45 cells had 10 ul Apr and the pMS82 cells were inoculated with 10 ul Hyg in LB -NaCl as Hygromycin is salt-intolerant.
500 ml SFM + 10 ml MgCl2???




Revision as of 02:16, 4 September 2013

Week Thirteen <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Outreach

Today we held our public engagement event at the forum in Norwich city centre. six members of our team attended the event which lasted all day. There were flags in maps of Europe and Norfolk to highlight where we had received soil samples from and freebies of pens, sweets and general science information. We had various posters and leaflets to explain about iGEM and our project.
The main attraction was biscuit decorating, which involved recreating a petri dish of bacteria on a biscuit using icing and sweets. We could then explain to the children and their parents about Streptomyces and antibiotic resistance. It was a very successful event with alot of interest.

Bacteria Biscuit making at Forum event




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