IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/30
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Floor OnePerformed 38 more Candida albicans overlays of Bioassays. Made up 2 x 200 ml SNA - 200 ml distilled water, 1.6 g nutrient broth powder and 1 g agar. This was autoclaved and left too cool in 55°C water bath (removed before molten stage). A 200 ul volume of Candida was added to 5 ml SNA per plate before swirling to disperse. Filtered, concentrated and resuspended more spore stocks in glycerol. Floor TwoThe ligation reactions from the previous day were retrieved and transformed onto LB agar + Chlorophenicol plates and left at room temperature for 48 hours. |