IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/03: Difference between revisions
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(Autocreate 2013/09/03 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
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== | ==Floor One== | ||
Made 1% agarose gel.<br> | |||
Filtered, concentrated and resuspended spore stocks in 20% glycerol.<br> | |||
Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products.<br> | |||
Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.<br> | |||
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br> | |||
Set up overnight cultures of the 36 spore stocks that produced anti-fungal compounds in the Bioassays. A 10 ul sample of spores was inoculated in 10 ml LB and incubated at 37 °C O/N. | |||
Revision as of 04:45, 25 September 2013
 iGEM Project name 1
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Floor OneMade 1% agarose gel.
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