IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/03: Difference between revisions
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Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.<br> | Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.<br> | ||
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br> | Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br> | ||
Set up overnight cultures of the 36 spore stocks that produced anti-fungal compounds in the Bioassays. A 10 ul sample of spores was inoculated in 10 ml LB and incubated at 37 °C O/N. | Set up overnight cultures of the 36 spore stocks that produced anti-fungal compounds in the Bioassays (examples in ''fig 2''). A 10 ul sample of spores was inoculated in 10 ml LB and incubated at 37 °C O/N. | ||
[[Image:Example.jpg]] | |||
Revision as of 15:08, 29 September 2013
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Floor OneMade 1% agarose gel.
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