IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/03: Difference between revisions
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==Floor One== | ==Floor One== | ||
Made 1% agarose gel.<br> | Made 1% agarose gel.<br> | ||
Filtered, concentrated and resuspended spore stocks in 20% glycerol.<br> | Filtered, concentrated and resuspended spore stocks in 20% glycerol.<br> | ||
Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products.<br> | Performed agarose gel electrophoresis on NdeI and HindIII pMS82 digest products ''fig 1''.<br> | ||
Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.<br> | Performed a gel extraction using a QIAquick kit on the top (undigested pMS82) and middle (unsure) bands.<br> | ||
Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br> | Set up 4 ligation reactions with the 2 extracted vectors, each with and without ligase.<br> | ||
Revision as of 09:48, 29 September 2013
 iGEM Project name 1
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Floor OneMade 1% agarose gel.
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