IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/09/04: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/09/04 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
 
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
==Entry title==
==Floor One==
* Insert your content here.
Performed chromosomal DNA preps for 12 of the O/N cultures that appeared to lack contaminants and had large areas of clearance in the Bioassays.<br>
The ligated vector (pMS82 with GUS insert) was transformed into chemically-competent <i>E. coli</i> cells through heat shock. The cells were then plated onto LB-NaCl (with 200 μL Hygromycin per 200 ml LB). There were 2 plates with 100 μL and 800 μL volumes plated respectively per ligation reaction.




Latest revision as of 23:16, 26 September 2017

iGEM Project name 1 Main project page
Previous entry      Next entry

Floor One

Performed chromosomal DNA preps for 12 of the O/N cultures that appeared to lack contaminants and had large areas of clearance in the Bioassays.
The ligated vector (pMS82 with GUS insert) was transformed into chemically-competent E. coli cells through heat shock. The cells were then plated onto LB-NaCl (with 200 μL Hygromycin per 200 ml LB). There were 2 plates with 100 μL and 800 μL volumes plated respectively per ligation reaction.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM/2013/09/04&oldid=1013182"