IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/06/24: Difference between revisions
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Jessica Gray (talk | contribs) (Autocreate 2014/06/24 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
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== | ==Week 2 Day 2== | ||
TSL | |||
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- Selected a single colony from each of the eleven plates and the colonies were grown in TY broth with Kanamycin O/N | |||
- Transformation of pSB1C3 plasmid containing RFP into E.coli using electroporation. Incubated with 50 μl of Soc broth at 37 °C for 1 hour. | |||
- Poured 11 agar plates containing chloramphenicol | |||
- Spreadplated E.coli cells which have been transformed with the pSB1C3 + RFP plasmid | |||
- Completed calculations for Dig-Lig experiments (Size and conc. of DNA parts calculated,size of acceptor calculated, order of constructs for each reaction determined, dilution and DNA conc. for each reaction determined) Ratio of 2:1, constructs:acceptor (plasmid) | |||
Revision as of 07:07, 9 July 2014
 iGEM Project name 1
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Week 2 Day 2TSL - Selected a single colony from each of the eleven plates and the colonies were grown in TY broth with Kanamycin O/N - Transformation of pSB1C3 plasmid containing RFP into E.coli using electroporation. Incubated with 50 μl of Soc broth at 37 °C for 1 hour. - Poured 11 agar plates containing chloramphenicol - Spreadplated E.coli cells which have been transformed with the pSB1C3 + RFP plasmid - Completed calculations for Dig-Lig experiments (Size and conc. of DNA parts calculated,size of acceptor calculated, order of constructs for each reaction determined, dilution and DNA conc. for each reaction determined) Ratio of 2:1, constructs:acceptor (plasmid)
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