IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/06/26
From OpenWetWare
 iGEM Project name 1
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Week 2 Day 4TSL - Checked Dig-Lig tranformation plates grown O/N and poor blue white selection observed with many blue colonies. - Selected 2 white colonies from each plated and colony PCR performed. - Following colony PCR, amplified fragments ran on gel to determine size and therefore whether they contained the construct. - Decided to repeat the 11 Dig-Lig experiments - Ran digested PCR product from 25.06.2014 on a diagnostic 1% agarose gel in TBE buffer and determined that LacZ had internal restriction enzyme cut sites and product was split into two bands.
| |
