IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/03: Difference between revisions

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(Autocreate 2014/07/03 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
 
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==Entry title==
==Entry title==
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TSL
----
- Set up a 20 µl PCR reaction using primers synthesized from Sigma and a level 1 acceptor as the template DNA
  • Pro + 5UTR
  • CDS
  • Ter + 3UTR
-Ran PCR product on a gel to check amplification
-PCR product purification using phenol chloroform





Revision as of 07:32, 24 July 2014

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Entry title

TSL


- Set up a 20 µl PCR reaction using primers synthesized from Sigma and a level 1 acceptor as the template DNA

 • Pro + 5UTR
 • CDS
 • Ter + 3UTR

-Ran PCR product on a gel to check amplification -PCR product purification using phenol chloroform