IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/03: Difference between revisions
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- Set up a 20 µl PCR reaction using primers synthesized from Sigma and a level 1 acceptor as the template DNA | - Set up a 20 µl PCR reaction using primers synthesized from Sigma and a level 1 acceptor as the template DNA | ||
• Pro + 5UTR | |||
• CDS | |||
• Ter + 3UTR | |||
-Ran PCR product on a gel to check amplification | -Ran PCR product on a gel to check amplification | ||
-PCR product purification using phenol chloroform | -PCR product purification using phenol chloroform | ||
Revision as of 07:33, 24 July 2014
 iGEM Project name 1
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Entry titleTSL - Set up a 20 µl PCR reaction using primers synthesized from Sigma and a level 1 acceptor as the template DNA • Pro + 5UTR • CDS • Ter + 3UTR -Ran PCR product on a gel to check amplification -PCR product purification using phenol chloroform
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