IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/22: Difference between revisions

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(Autocreate 2014/07/22 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM)
 
 
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==Week 6 Day 2==
|-
 
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
- Transformation of new DNA from DNA 2.0 into ''E.coli''. Tubes 1 to 7.
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|-
1. AtPR1
| colspan="2"|
 
==Entry title==
2. ArvBS3
* Insert your content here.
 
3. NbBi1-RNA
 
4. NbProB-RN
 
5. AvrXa27 T
 
6. OsXa27 Pro
 
7. Control (No DNA)
 
- Followed heat shock protocol:
 
- 5 µl DNA added to 50 µl of chemically competent ''E.coli''
 
- Spreadplated onto agar plates containing KAN
 
- Incubated O/N at 37°C
 
 
Human Practices
 
----
 
Met with Dr Kay Yeoman, BIO outreach officer to discuss activities for school outreach activities
 
Discussed activities for 'Food for Thought' event at the cut on 26/07






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Latest revision as of 07:32, 24 July 2014

Week 6 Day 2

- Transformation of new DNA from DNA 2.0 into E.coli. Tubes 1 to 7.

1. AtPR1

2. ArvBS3

3. NbBi1-RNA

4. NbProB-RN

5. AvrXa27 T

6. OsXa27 Pro

7. Control (No DNA)

- Followed heat shock protocol:

- 5 µl DNA added to 50 µl of chemically competent E.coli

- Spreadplated onto agar plates containing KAN

- Incubated O/N at 37°C


Human Practices

Met with Dr Kay Yeoman, BIO outreach officer to discuss activities for school outreach activities

Discussed activities for 'Food for Thought' event at the cut on 26/07



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