IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/22: Difference between revisions

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==Week 6 Day 2==
|-
 
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
- Transformation of new DNA from DNA 2.0 into ''E.coli''. Tubes 1 to 7.
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|-
1. AtPR1
| colspan="2"|
 
2. ArvBS3
 
3. NbBi1-RNA
 
4. NbProB-RN
 
5. AvrXa27 T
 
6. OsXa27 Pro
 
7. Control (No DNA)
 
- Followed heat shock protocol:
 
- 5 µl DNA added to 50 µl of chemically competent ''E.coli''
 
- Spreadplated onto agar plates containing KAN
 
- Incubated O/N at 37°C
 
 
Human Practices
Human Practices
----
Met with Dr Kay Yeoman, BIO outreach officer to discuss activities for school outreach activities
Met with Dr Kay Yeoman, BIO outreach officer to discuss activities for school outreach activities
Discussed activities for 'Food for Thought' event at the cut on 26/07
Discussed activities for 'Food for Thought' event at the cut on 26/07


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Latest revision as of 07:32, 24 July 2014

Week 6 Day 2

- Transformation of new DNA from DNA 2.0 into E.coli. Tubes 1 to 7.

1. AtPR1

2. ArvBS3

3. NbBi1-RNA

4. NbProB-RN

5. AvrXa27 T

6. OsXa27 Pro

7. Control (No DNA)

- Followed heat shock protocol:

- 5 µl DNA added to 50 µl of chemically competent E.coli

- Spreadplated onto agar plates containing KAN

- Incubated O/N at 37°C


Human Practices

Met with Dr Kay Yeoman, BIO outreach officer to discuss activities for school outreach activities

Discussed activities for 'Food for Thought' event at the cut on 26/07



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