IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/22: Difference between revisions
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Human Practices | |||
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Latest revision as of 07:32, 24 July 2014
Week 6 Day 2
- Transformation of new DNA from DNA 2.0 into E.coli. Tubes 1 to 7.
1. AtPR1
2. ArvBS3
3. NbBi1-RNA
4. NbProB-RN
5. AvrXa27 T
6. OsXa27 Pro
7. Control (No DNA)
- Followed heat shock protocol:
- 5 µl DNA added to 50 µl of chemically competent E.coli
- Spreadplated onto agar plates containing KAN
- Incubated O/N at 37°C
Human Practices
Met with Dr Kay Yeoman, BIO outreach officer to discuss activities for school outreach activities
Discussed activities for 'Food for Thought' event at the cut on 26/07
