IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/08/04: Difference between revisions
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Jessica Gray (talk | contribs) (Autocreate 2014/08/04 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
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== | ==Week 8 Day 1== | ||
- Colony PCR of Agrobacterium plates containing constructs 2,3,4,10,11 which had been grown over the weekend at 28 °C. | |||
- Colony PCR products run on a 1% agarose TAE gel with a DNA positive control. | |||
- Picked single colonies of Level 1 constructs: 1, 5, 9, 15, 16, 17, 18, 19. Colony PCR of these level 1 constructs. | |||
- 2 Agrobacterium colonies selected from each plate to be grown in 5 ml of LB, 100 µl Ampicillin, 200 µl Gentamycin and 200 µl Rifampicin O/N at 28 °C with shaking. | |||
Human Practices | |||
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- 93 agar plates autoclaved and poured for Human Practices school events in September, kept in cold room. | |||
Revision as of 08:48, 4 August 2014
 iGEM Project name 1
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Week 8 Day 1- Colony PCR of Agrobacterium plates containing constructs 2,3,4,10,11 which had been grown over the weekend at 28 °C. - Colony PCR products run on a 1% agarose TAE gel with a DNA positive control. - Picked single colonies of Level 1 constructs: 1, 5, 9, 15, 16, 17, 18, 19. Colony PCR of these level 1 constructs. - 2 Agrobacterium colonies selected from each plate to be grown in 5 ml of LB, 100 µl Ampicillin, 200 µl Gentamycin and 200 µl Rifampicin O/N at 28 °C with shaking.
Human Practices - 93 agar plates autoclaved and poured for Human Practices school events in September, kept in cold room.
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