IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/08/08

From OpenWetWare
Jump to navigationJump to search
iGEM Project name 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Week 8 Day 4

  • Spread the level 2 constructs A-G on spectinomycin plates with IPTG and XGAL for blue white selection as well as plating the transformation of vector pAGM8031.
  • Plated end linkers 2 and 3 on ampicillin
  • Picked colonies of Agrobacterium tumerfaciens, 2, 3, 4, 10 and 11 and grew in an overnight culture with shaking with LB and ampicillin.
Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM/2014/08/08&oldid=805810"