IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/06/05: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week One </span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week One </span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Today was the first day of Lab work taking place on floor two. We were able to test the efficiency of our competent cells using a kit supplied | Today was the first day of Lab work taking place on floor two. We were able to test the efficiency of our competent cells using a kit supplied | ||
by iGEM. We performed transformations into our α-Select competent cells using the part BBa_J04450 DNA in pSB1C3 plasmids of three concentrations: 0.5,10 and 50pg/μL. We then plated the samples onto Agar plates containing Chloramphenicol and incubated overnight. | by iGEM. We performed transformations into our α-Select competent cells using the part BBa_J04450 DNA in pSB1C3 plasmids of three concentrations: 0.5,10 and 50pg/μL. We then plated the samples onto Agar plates containing Chloramphenicol and incubated overnight. | ||
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Outreach | ==Outreach== | ||
We have applied for some marine sediment (south China sea, North Atlantic) samples from a French environmental studies university. | We have applied for some marine sediment (south China sea, North Atlantic) samples from a French environmental studies university. |
Latest revision as of 22:45, 26 September 2017
Week One | Main project page Next entry |
Transformation Efficiency KitToday was the first day of Lab work taking place on floor two. We were able to test the efficiency of our competent cells using a kit supplied by iGEM. We performed transformations into our α-Select competent cells using the part BBa_J04450 DNA in pSB1C3 plasmids of three concentrations: 0.5,10 and 50pg/μL. We then plated the samples onto Agar plates containing Chloramphenicol and incubated overnight. image? OutreachWe have applied for some marine sediment (south China sea, North Atlantic) samples from a French environmental studies university. If successful we will also have to use the sediments to teach a summer school class of 10 GCSE students. Tuesday 16th July 14.00 – 14.30 Talk about iGEM to summer school students
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