IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/02: Difference between revisions
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==Floor One== | |||
Spore stock preparation, filtering and concentrating (spinning down at 13 000 rpm for 1 min and pouring off supernatant) was continued. | |||
The conjugations were overlayed with 1 ml sterile water, 20 ul nalidixic acid and 100 ul kanamycin per plate. The plates were then incubated again overnight at 30 °C. | |||
N.B. These plates were accidentally left out at room temp. over the weekend so it is uncertain whether they will work - the E. coli may have had a chance to outcompete the Strep. (?). | |||
Streak-purified several contaminated single colony Streptomyces plates. | |||
==Floor Two== | ==Floor Two== | ||
The two cultures for the protein expression of σ AntA were retrieved from the incubator. 1.5ml was taken from each and centrifuged for five minutes. The supernatant was removed from each sample and the remaining pellets were stored in the freezer at -20°C. | The two cultures for the protein expression of σ AntA were retrieved from the incubator. 1.5ml was taken from each and centrifuged for five minutes. The supernatant was removed from each sample and the remaining pellets were stored in the freezer at -20°C. | ||
Revision as of 10:05, 15 August 2013
 Week Nine
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Floor OneSpore stock preparation, filtering and concentrating (spinning down at 13 000 rpm for 1 min and pouring off supernatant) was continued. The conjugations were overlayed with 1 ml sterile water, 20 ul nalidixic acid and 100 ul kanamycin per plate. The plates were then incubated again overnight at 30 °C. N.B. These plates were accidentally left out at room temp. over the weekend so it is uncertain whether they will work - the E. coli may have had a chance to outcompete the Strep. (?). Streak-purified several contaminated single colony Streptomyces plates.
Floor TwoThe two cultures for the protein expression of σ AntA were retrieved from the incubator. 1.5ml was taken from each and centrifuged for five minutes. The supernatant was removed from each sample and the remaining pellets were stored in the freezer at -20°C. OutreachThe Hotel for the European Jamboree in Lyon was booked.
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