IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/06: Difference between revisions
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(Autocreate 2013/08/06 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Floor One== | ||
Made 1 % agarose gel - 2 x large - 2 x 100 ml TBE buffer and 1 g agarose. Melted in microwave til agarose dissolved. | |||
Prepared spore stocks of all lawns. | |||
Did as much lawning and streak purifying as was possile with available SFM + Ny media. | |||
Made 6 x 500 ml SFM agar, autoclaved for 1 hr and poured into plates. | |||
Loaded and ran gels - added 4 ul loading dye to each sample (46). Used 2 x ladder (1 Kb). | |||
Made up more liquid Nystatin (50 mg/ml) - 10 ml DMSO and 500 mg Nystatin. | |||
Latest revision as of 23:11, 26 September 2017
iGEM Project name 1 | Main project page Previous entry Next entry |
Floor OneMade 1 % agarose gel - 2 x large - 2 x 100 ml TBE buffer and 1 g agarose. Melted in microwave til agarose dissolved. Prepared spore stocks of all lawns. Did as much lawning and streak purifying as was possile with available SFM + Ny media. Made 6 x 500 ml SFM agar, autoclaved for 1 hr and poured into plates. Loaded and ran gels - added 4 ul loading dye to each sample (46). Used 2 x ladder (1 Kb). Made up more liquid Nystatin (50 mg/ml) - 10 ml DMSO and 500 mg Nystatin.
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