IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/09: Difference between revisions
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==Floor two== | ==Floor two== | ||
Another restriction digest of all the biobricks was prepared with different enzymes, to produce larger fragments. We used the enzymes PvuII and Xho1 to enable us to distinguish between the biobricks as the digests, when analysed using agarose gel electorphoresis, produced different size fragments for each plasmid. A sample was also cut with EcoRI and PstI ''fig 2,3''. | Another restriction digest of all the biobricks was prepared with different enzymes, to produce larger fragments. We used the enzymes PvuII and Xho1 to enable us to distinguish between the biobricks as the digests, when analysed using agarose gel electorphoresis, produced different size fragments for each plasmid. A sample was also cut with EcoRI and PstI ''fig 2,3''. <br> | ||
The plasmid Pet28AntA which produces the σ factor AntA was transformed into BL21 and BL21 star competent ''E.coli''cells. The four plates were left to incubate over the weekend at room temperature. | |||
[[Image:BIORAD 2013-08-09 16hr 21min.jpg|thumb|Fig 2. Analysis of restriction digest of biobricks using enzymes PvuII and XhoI. Lanes 1,3,5,7,9 contain samples 3b2,1 colony 1,1 colony 2, 2 colony 1, 2 colony 2 respectively, which were cut with PvuII. Lanes 2,4,6,8,10 contain samples 3b2, 1 colony 1, 1 colony 2, 2 colony 1, 2 colony 2 respectively, which were cut with XhoI.]] | [[Image:BIORAD 2013-08-09 16hr 21min.jpg|thumb|Fig 2. Analysis of restriction digest of biobricks using enzymes PvuII and XhoI. Lanes 1,3,5,7,9 contain samples 3b2,1 colony 1,1 colony 2, 2 colony 1, 2 colony 2 respectively, which were cut with PvuII. Lanes 2,4,6,8,10 contain samples 3b2, 1 colony 1, 1 colony 2, 2 colony 1, 2 colony 2 respectively, which were cut with XhoI.]] | ||
[[Image:BIORAD 2013-08-09 16hr 23min.jpg|thumb|Fig 3. Analysis of restriction digest of biobricks using enzymes PvuII, XhoI, EcoRI and PstI. Lanes 1,3,5,7, contain samples 2b1,2b2,2c1,2c2 respectively, which were cut with PvuII. Lanes 2,4,6,8, contain samples 2b1,2b2,2c1,2c2 respectively, which were cut with XhoI. Lanes 10,11,12 contain sample 3c cut with EcoRI, PstI and No enzyme respectively]] | [[Image:BIORAD 2013-08-09 16hr 23min.jpg|thumb|Fig 3. Analysis of restriction digest of biobricks using enzymes PvuII, XhoI, EcoRI and PstI. Lanes 1,3,5,7, contain samples 2b1,2b2,2c1,2c2 respectively, which were cut with PvuII. Lanes 2,4,6,8, contain samples 2b1,2b2,2c1,2c2 respectively, which were cut with XhoI. Lanes 10,11,12 contain sample 3c cut with EcoRI, PstI and No enzyme respectively]] | ||
Revision as of 02:30, 15 August 2013
 Week 10
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Floor twoAnother restriction digest of all the biobricks was prepared with different enzymes, to produce larger fragments. We used the enzymes PvuII and Xho1 to enable us to distinguish between the biobricks as the digests, when analysed using agarose gel electorphoresis, produced different size fragments for each plasmid. A sample was also cut with EcoRI and PstI fig 2,3.   | |
