IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/23: Difference between revisions
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==Floor Two== | ==Floor Two== | ||
Our focus of the experiments was to prepare more DNA of the confirmed biobricks and to reclone our fourth brick. <br> | Our focus of the experiments was to prepare more DNA of the confirmed biobricks and to reclone our fourth brick. <br> | ||
To prepare biobrick J04450+neo; plasmids 3b and AntGpneo were cut with restriction enzymes NdeI and PstI. The samples were ran on a agarose gel | To prepare biobrick J04450+neo; plasmids 3b and AntGpneo were cut with restriction enzymes NdeI and PstI. The samples were ran on a agarose gel, but the band were unclear so the gel was not used further. <br> | ||
DNA from 3a and BBa_J04450 were transformed into α-select gold competent cells and spread on LB agar plates with added chlorophenicol | DNA from 3a and BBa_J04450 were transformed into α-select gold competent cells and spread on LB agar plates with added chlorophenicol antibiotic and incubated overnight at 37°C. | ||
Revision as of 04:48, 29 August 2013
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Floor TwoOur focus of the experiments was to prepare more DNA of the confirmed biobricks and to reclone our fourth brick.
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