IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/30: Difference between revisions
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The ligation reactions from the previous day were retrieved and transformed onto LB agar + Chlorophenicol plates and left at room temperature for 48 hours. <br> | The ligation reactions from the previous day were retrieved and transformed onto LB agar + Chlorophenicol plates and left at room temperature for 48 hours. <br> | ||
Minipreps were performed on the inoculated cultures of samples 3a and Bba_J04450 and left in the -20°C freezer. <br> | Minipreps were performed on the inoculated cultures of samples 3a and Bba_J04450 and left in the -20°C freezer. <br> | ||
The pellets of BL21 cells with | The pellets of BL21 cells with pET28AntA plasmid and BL21 cells with pET28AntA and AntGRFP plasmids were retrieved. The soluble and insoluble fractions were prepared and analysed on a 15% SDS-PAGE gel. | ||
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Revision as of 04:21, 11 September 2013
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Floor OnePerformed 38 more Candida albicans overlays of Bioassays. Made up 2 x 200 ml SNA - 200 ml distilled water, 1.6 g nutrient broth powder and 1 g agar. This was autoclaved and left too cool in 55°C water bath (removed before molten stage). A 200 ul volume of Candida was added to 5 ml SNA per plate before swirling to disperse. Floor TwoThe ligation reactions from the previous day were retrieved and transformed onto LB agar + Chlorophenicol plates and left at room temperature for 48 hours. |