IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/30: Difference between revisions
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(Autocreate 2013/08/30 Entry for IGEM:University_of_East_Anglia_(UEA),_Norwich,_UK/2009/Notebook/NRP-UEA-Norwich_iGEM) |
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== | ==Floor One== | ||
Performed 38 more Candida albicans overlays of Bioassays. Made up 2 x 200 ml SNA - 200 ml distilled water, 1.6 g nutrient broth powder and 1 g agar. This was autoclaved and left too cool in 55°C water bath (removed before molten stage). A 200 ul volume of Candida was added to 5 ml SNA per plate before swirling to disperse.<br> | |||
Agarose gel electrophoresis was performed on the gradient PCR products (110 mV for 35 min). The GUS gene was visible in all reactions at approx. 1.8 Kb.<br> | |||
Filtered, concentrated and resuspended more spore stocks in glycerol.<br> | |||
Performed a QIAquick gel extraction of gel to retrieve the new GUS gene.<br> | |||
Re-streaked the conjugations (S4, S4 △antA) onto SFM (+ Ny) with Thiostrepton. | |||
Revision as of 04:42, 3 September 2013
 iGEM Project name 1
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Floor OnePerformed 38 more Candida albicans overlays of Bioassays. Made up 2 x 200 ml SNA - 200 ml distilled water, 1.6 g nutrient broth powder and 1 g agar. This was autoclaved and left too cool in 55°C water bath (removed before molten stage). A 200 ul volume of Candida was added to 5 ml SNA per plate before swirling to disperse.
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