IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2014/07/30: Difference between revisions
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== | ==Week 7 Day 3== | ||
- Picked 1 white colony from 29/07/14 transformed dig-lig plates and added to 5 ml LB + 5 μl Ampicillin and incubated at 37 °C also added to 50 μl of PCR Master Mix containing: | |||
1 μl DNTPs | |||
2 μl Primer 1 | |||
2 μl Primer 2 | |||
5 μl Buffer | |||
2 μl MgCl2 | |||
0.5 μl BIOTAQ Enzyme | |||
37 μl Water | |||
- PCR programme: | |||
Step 1 : 95 °C 5 min | |||
Step 2 : 95 °C 1 min | |||
Step 3 : 54 °C 1.5 min | |||
Step 4 : 72 °C 1 min | |||
Step 5 : 72 °C 5 min | |||
Steps 2, 3, 4 repeated x 34 times | |||
- Run on a 1 % agarose TAE gel at 100V. Each well loaded with 2 μl of loading dye and 20 μl of DNA. Two 1kb ladders loaded. | |||
- DNA mini prep of cultures from 29/07/14 (DNA 2.0 level 0's and PRO, CDS, TER) (2.5 mls) | |||
- Glycerol stocks made from 1 ml of O/N culture. | |||
- Transformations of DNA iGEM #2-19 and Vectors #1-4 intro E.coli to generate more DNA. | |||
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Week 7 Day 3- Picked 1 white colony from 29/07/14 transformed dig-lig plates and added to 5 ml LB + 5 μl Ampicillin and incubated at 37 °C also added to 50 μl of PCR Master Mix containing: 1 μl DNTPs 2 μl Primer 1 2 μl Primer 2 5 μl Buffer 2 μl MgCl2 0.5 μl BIOTAQ Enzyme 37 μl Water - PCR programme: Step 1 : 95 °C 5 min Step 2 : 95 °C 1 min Step 3 : 54 °C 1.5 min Step 4 : 72 °C 1 min Step 5 : 72 °C 5 min Steps 2, 3, 4 repeated x 34 times - Run on a 1 % agarose TAE gel at 100V. Each well loaded with 2 μl of loading dye and 20 μl of DNA. Two 1kb ladders loaded. - DNA mini prep of cultures from 29/07/14 (DNA 2.0 level 0's and PRO, CDS, TER) (2.5 mls) - Glycerol stocks made from 1 ml of O/N culture. - Transformations of DNA iGEM #2-19 and Vectors #1-4 intro E.coli to generate more DNA. |