IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/06/05

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(Transformation Efficiency Kit)
Current revision (04:01, 19 July 2013) (view source)
 
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Day One </span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week One </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Transformation Efficiency Kit==
==Transformation Efficiency Kit==
Today was the first day of Lab work taking place on floor two. We were able to test the efficiency of our competent cells using a kit supplied  
Today was the first day of Lab work taking place on floor two. We were able to test the efficiency of our competent cells using a kit supplied  
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by iGEM.   
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by iGEM. We performed transformations into our α-Select competent cells using the part BBa_J04450 DNA in pSB1C3 plasmids of three concentrations: 0.5,10 and 50pg/μL. We then plated the samples onto Agar plates containing Chloramphenicol and incubated overnight.   
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image?
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==Outreach==
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We have applied for some marine sediment (south China sea, North Atlantic) samples from a French environmental studies university.
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If successful we will also have to use the sediments to teach a summer school class of 10 GCSE students.
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Tuesday 16th July 14.00 – 14.30 Talk about iGEM to summer school students

Current revision

Week One Main project page
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Transformation Efficiency Kit

Today was the first day of Lab work taking place on floor two. We were able to test the efficiency of our competent cells using a kit supplied by iGEM. We performed transformations into our α-Select competent cells using the part BBa_J04450 DNA in pSB1C3 plasmids of three concentrations: 0.5,10 and 50pg/μL. We then plated the samples onto Agar plates containing Chloramphenicol and incubated overnight. image?

Outreach

We have applied for some marine sediment (south China sea, North Atlantic) samples from a French environmental studies university. If successful we will also have to use the sediments to teach a summer school class of 10 GCSE students.

Tuesday 16th July 14.00 – 14.30 Talk about iGEM to summer school students



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