IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/06/21

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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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The restriction digests were retrieved from the freezer while a gel was set up. After the gel had ran for 2 hours, the bands of DNA were visible under ultra violet light so the digest had worked. This meant we could go ahead and remove the fragments of interest and isolate the DNA using a gel cleanup system yielding several samples including four of large plasmid, two of extra large plasmid and one of antGP.  
The restriction digests were retrieved from the freezer while a gel was set up. After the gel had ran for 2 hours, the bands of DNA were visible under ultra violet light so the digest had worked. This meant we could go ahead and remove the fragments of interest and isolate the DNA using a gel cleanup system yielding several samples including four of large plasmid, two of extra large plasmid and one of antGP.  
The next stage involved ligation reactions of various different combinations of iGEM plasmid and antGP fragments using the isolated DNA fragments from both today and 19th June. The samples were left to incubate at 4°C overnight.   
The next stage involved ligation reactions of various different combinations of iGEM plasmid and antGP fragments using the isolated DNA fragments from both today and 19th June. The samples were left to incubate at 4°C overnight.   

Revision as of 06:45, 25 June 2013

The restriction digests were retrieved from the freezer while a gel was set up. After the gel had ran for 2 hours, the bands of DNA were visible under ultra violet light so the digest had worked. This meant we could go ahead and remove the fragments of interest and isolate the DNA using a gel cleanup system yielding several samples including four of large plasmid, two of extra large plasmid and one of antGP. The next stage involved ligation reactions of various different combinations of iGEM plasmid and antGP fragments using the isolated DNA fragments from both today and 19th June. The samples were left to incubate at 4°C overnight.
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