IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/06/26

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Four</span>
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Cultures grown overnight were retrieved and DNA mini preps was performed. This was followed by restriction digest reactions using EcoR1 and Pst1 enzymes. Samples were then run on an agarose gel to confirm the digest reactions.
Cultures grown overnight were retrieved and DNA mini preps was performed. This was followed by restriction digest reactions using EcoR1 and Pst1 enzymes. Samples were then run on an agarose gel to confirm the digest reactions.
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[[Image:26-6-2013.jpg |right|300px]]
Also, it was today in which we discovered and confirmed that our AntgP Neo sequence was wrong, missing the Xba1 restriction site. So we had to do further planning for potential experiments.
Also, it was today in which we discovered and confirmed that our AntgP Neo sequence was wrong, missing the Xba1 restriction site. So we had to do further planning for potential experiments.

Revision as of 18:39, 17 July 2013

Week Four Main project page
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Cultures grown overnight were retrieved and DNA mini preps was performed. This was followed by restriction digest reactions using EcoR1 and Pst1 enzymes. Samples were then run on an agarose gel to confirm the digest reactions.

Also, it was today in which we discovered and confirmed that our AntgP Neo sequence was wrong, missing the Xba1 restriction site. So we had to do further planning for potential experiments.



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