IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/06/26
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Cultures grown overnight were retrieved and DNA mini preps was performed. This was followed by restriction digest reactions using EcoR1 and Pst1 enzymes. Samples were then run on an agarose gel to confirm the digest reactions.
Also, it was today in which we discovered and confirmed that our AntgP Neo sequence was wrong, missing the Xba1 restriction site. So we had to do further planning for potential experiments.
Fig:Analysis of Restriction Digest products of the ligated fragments cut with EcoR1 and Pst1 using agrose gel electrophoresis.