IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/11

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Cultures were retrieved from incubation and minipreps were performed following the protocol. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel to confirm the results.
Cultures were retrieved from incubation and minipreps were performed following the protocol. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel to confirm the results.
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[[Image:2013-07-11 cut sites confirmation.jpg|thumb|Fig. Analysis of restriction digest. controls of uncut Antgp and J04450 plasmids in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain samples 2a,2b,2c,3a,3b,3c respectively.]]  
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[[Image:2013-07-11 cut sites confirmation.jpg|thumb|Fig. Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c with Nde1 respectively.]]  
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The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.
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The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.
 
==Outreach==
==Outreach==
As the UK meet up was the following day, we practiced and finalised our presentation.  
As the UK meet up was the following day, we practiced and finalised our presentation.  

Revision as of 09:41, 31 July 2013

Week Six Main project page
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Lab

Cultures were retrieved from incubation and minipreps were performed following the protocol. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel to confirm the results.

Fig. Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c  with Nde1 respectively.
Fig. Analysis of restriction digest. Antgp plasmid cut with Xba1 and J04450 plasmid cut with Nde1 in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain digested samples 2a,2b,2c, with Xba1 3a,3b,3c with Nde1 respectively.

The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.

Outreach

As the UK meet up was the following day, we practiced and finalised our presentation.



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