IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/11
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SH Cultures were retrieved from incubation and minipreps were performed following the protocol. This was followed by a restriction enzyme digest reaction using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel to confirm the results. LC The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.
As the UK meet up was the following day, we practiced and finalised our presentation.