IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/11

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Lab

Cultures were retrieved from incubation and minipreps were performed following the protocol. This was followed by a restriction enzyme digest reactions using Xba1 and Nde1 enzymes each relevant to its DNA .After incubation samples were run on a gel to confirm the results.

Fig. Analysis of restriction digest. controls of uncut Antgp and J04450 plasmids in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain samples 2a,2b,2c,3a,3b,3c respectively.
Fig. Analysis of restriction digest. controls of uncut Antgp and J04450 plasmids in lanes 1 and 2 respectively. Lanes 3-8 contain uncut samples 2a,2b,2c,3a,3b,3c respectively . Lanes 10-15 contain samples 2a,2b,2c,3a,3b,3c respectively.

The four cultures for the protein expression were recovered and centrifuged to provide a pellet, yielding eight pellets, two from each culture.

Outreach

As the UK meet up was the following day, we practiced and finalised our presentation.



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