IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/07/17

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===Floor Two===
===Floor Two===
Today's experiments were not totally relevant to the project. But because of our suspicions that our enzymes are not working properly, we decided to test them on J04450 and another plasmid called pSB, while waiting for the sequencing results to arrive. We teted Pst1, Nde1 and Xba1 enzymes.
Today's experiments were not totally relevant to the project. But because of our suspicions that our enzymes are not working properly, we decided to test them on J04450 and another plasmid called pSB, while waiting for the sequencing results to arrive. We teted Pst1, Nde1 and Xba1 enzymes.
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To continue protein expression the half the pellets (1a,1b,2a,2b) were resuspended, sonicated and centrifuged before the supernatant of each sample was seperated into another tube. The pellets that remained were resuspended and the samples were stored in the freezer.
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Revision as of 08:27, 19 July 2013

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Lab

Floor Two

Today's experiments were not totally relevant to the project. But because of our suspicions that our enzymes are not working properly, we decided to test them on J04450 and another plasmid called pSB, while waiting for the sequencing results to arrive. We teted Pst1, Nde1 and Xba1 enzymes. To continue protein expression the half the pellets (1a,1b,2a,2b) were resuspended, sonicated and centrifuged before the supernatant of each sample was seperated into another tube. The pellets that remained were resuspended and the samples were stored in the freezer.


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